Oxidation and amination of primary alcohols

摘要

A method including the steps a) providing a primary alcohol of the formula HO—(CH2)x—R1, where R1 is —OH, —SH, —NH2 or —COOR2; x is at least 3; and R2 is H, alkyl or aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)+-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, where the NAD(P)+-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme. A whole cell catalyst for carrying out the method. The use of such a whole cell catalyst for oxidizing a primary alcohol.

主权项

1. A method for producing an amine or a diamine, comprising:
(a) contacting a primary alcohol of the formula
HO—(CH)—R1,with an NAD(P)+-dependent alcohol dehydrogenase to oxidize said primary alcohol to form an aldehyde or ketone oxidation product, wherein R1 is selected from the group consisting of —OH, —SH, —NH2 and —COOR2, x is at least 3 and R2 is selected from the group consisting of H, alkyl and aryl; and (b) contacting said aldehyde or ketone oxidation product with a transaminase to transfer α-amino groups from an amino acid to an α-ketocarboxylic acid to produce said amine or said diamine, wherein said NAD(P)+-dependent alcohol dehydrogenase, said transaminase, or both, are a recombinant or isolated enzyme.