摘要 |
The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such as Trichoderma cells, wherein at least 90% (mol%), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii.a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing α1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein. |
申请人 |
Glykos Finland OY |
发明人 |
Natunen, Jari;Leppanen, Anne;Salminen, Heidi;Hiltunen, Jukka;Kanerva, Anne;Heiskanen, Annamari;Westerholm-Parvinen, Ann;Schmidt, Georg;Huuskonen, Anne;Mustalahti, Eero;Landowski, Christopher;Saloheimo, Markku;Saarinen, Juhani;Sommer, Benjamin Patrick;Wahl, Ramon;Ostermeier, Christian;Helk, Bernhard |