Production of trichoderma citrinoviride micropropagules as a biocontrol agent by means of an economical process

摘要

The present invention relates to producing micropropagules from Trichoderma citrinoviride EGE-K-130 mold strain isolated from resources of our country and whose strain is molecularly identified, as a bio-control agent by means of an economical production process.

主权项

1. A process for preparing micropropagules as a bio-control agent from Trichoderma citrinoviride EGE-K-130 mould strain isolated from timber wastes in a plurality of trays, wherein the trays include an inner tray and an outer tray, both made of stainless steel, and the inner tray includes perforations on a bottom and sides of the inner tray, the process comprising:
adjusting an initial humidity ratio of solid media to be in a range of 60-75%, wherein the media is provided at an amount of 200-300 g, including 1-10% malt grass and 80-99% wheat bran by weight; adjusting the initial pH value of the media to be in the range of 4.00-6.00, using a 5NHCl solution; dispersing the solid media onto a perforated inner tray; holding the perforated inner tray by the handles; placing the perforated inner tray into the outer tray; sterilizing at a temperature of 121° C. for 30 minutes in an autoclave; multiplying a plurality of spores in potato dextrose agar (PDA); preparing a spore suspension at an amount of 1.0×102 spores/ml; inoculating the spore suspension into the media; producing the micropropagules on the inner tray; determining micropropagules quantity during and after the process.