发明名称 |
Hyperactive piggybac transposases |
摘要 |
The present invention provides PiggyBac transposase proteins, nucleic acids encoding the same, compositions comprising the same, kits comprising the same, non-human transgenic animals comprising the same, and methods of using the same. |
申请公布号 |
US9546382(B2) |
申请公布日期 |
2017.01.17 |
申请号 |
US201313774718 |
申请日期 |
2013.02.22 |
申请人 |
Poseida Therapeutics, Inc. |
发明人 |
Ostertag Eric;Madison Blair |
分类号 |
A01K67/00;C12N15/85;A61K48/00;C12N9/22;A61D19/04;A01K67/033;A01K67/027;C12N15/90 |
主分类号 |
A01K67/00 |
代理机构 |
Cooley LLP |
代理人 |
Cooley LLP ;Elrifi Ivor R.;Miller Katherine J. |
主权项 |
1. A non-human, transgenic animal whose genome comprises a nucleic acid molecule encoding a protein comprising at least 90% sequence identity to SEQ ID NO:2, and comprising at least one of the following amino acid substitutions in SEQ ID NO:2: a serine for the alanine at position 46; a threonine for the alanine at position 46; a proline for the arginine at position 119; an alanine for the cysteine at position 125; a leucine for the cysteine at position 125; a lysine for the tyrosine at position 177; a histidine for the tyrosine at position 177; a leucine for the phenylalanine at position 180; an isoleucine for the phenylalanine at position 180; a valine for the phenylalanine at position 180; a leucine for the methionine at position 185; a glycine for the alanine at position 187; a tryptophan for the phenylalanine at position 200; a proline for the valine at position 207; a phenylalanine for the valine at position 209; a phenylalanine for the methionine at position 226; an arginine for the leucine at position 235; a lysine for the valine at position 240; a leucine for the phenylalanine at position 241; a lysine for the proline at position 243; a glutamine for the methionine at position 282; a tryptophan for the leucine at position 296; a tyrosine for the leucine at position 296; a phenylalanine for the leucine at position 296; a leucine for the methionine at position 298; an alanine for the methionine at position 298; a valine for the methionine at position 298; an isoleucine for the proline at position 311; a valine for the proline at position 311; a lysine for the arginine at position 315; a glycine for the threonine at position 319; an arginine for the tyrosine at position 327; a valine for the tyrosine at position 328; a glycine for the cysteine at position 340; a leucine for the cysteine at position 340; a histidine for the aspartic acid at position 421; an isoleucine for the valine at position 436; a tyrosine for the methionine at position 456; a phenylalanine for the leucine at position 470; a lysine for the serine at position 486; a leucine for the methionine at position 503; an isoleucine for the methionine at position 503; a lysine for the valine at position 552; or a threonine for the alanine at position 570, operably linked to a promoter, wherein the protein is expressed. |
地址 |
San Diego CA US |