Rapid LC Mapping of CoV Values for SelexION Technology Using MRM-Triggered MRM Functionality

摘要

MRM triggered MRM, where the triggered MRM transitions make use of mobility device parameter values for the same compound, is performed. A plurality of primary MRM transitions are received and stored together with a mobility device parameter value for each transition as an MRM cycle list. Control information instructs a mobility device and a mass spectrometer to interrogate each MRM transition on the MRM cycle list within an MRM cycle of the mass spectrometer. If a product ion intensity value of an MRM transition exceeds a threshold value for a primary MRM transition, a plurality of secondary MRM transitions of the primary MRM transition with different mobility device parameter values are added to the MRM cycle list. The intensities of the measured secondary MRM transitions provide information on the optimum mobility device parameter for each compound.

主权项

1. A system for performing multiple reaction monitoring (MRM) triggered MRM that triggers MRM transitions that use different mobility device parameter values for the same compound, comprising:
an ion source that ionizes a sample producing an ion beam; a mobility device that receives the ion beam from the ion source and is adapted to accept a mobility device parameter value that causes the mobility device to select ions from the ion beam based on the mobility device parameter value; a mass spectrometer that is adapted to receive selected ions from the mobility device and perform MRM scans on the received ions; and a processor in communication with the mass spectrometer and the mobility device that
(a) receives one or more MRM precursor ion to product ion transitions for one or more compounds from a user, producing a plurality of primary MRM transitions,(b) for each transition of the plurality of primary MRM transitions, creates and stores a plurality of secondary MRM precursor ion to product ion transitions that each include the same precursor ion to product ion transition as the primary MRM transition but have different mobility device parameter values,(c) creates and stores an MRM cycle list that initially includes the plurality of primary MRM transitions and an initial mobility device parameter value for each transition of the plurality of primary MRM transitions,(d) sends control information, including the MRM cycle list, to the mobility device and the mass spectrometer instructing the mobility device and the mass spectrometer to interrogate each MRM transition on the MRM cycle list within an MRM cycle of the mass spectrometer by, for each MRM transition, applying the mobility device parameter value to the mobility device and performing an MRM scan using the mass spectrometer,(e) receives a product ion intensity value from the mass spectrometer for each MRM transition and stores the product ion intensity value with a primary or secondary MRM transition represented by the MRM transition, and(f) if a product ion intensity value of an MRM transition exceeds a threshold value and the MRM transition is a primary MRM transition, adds a plurality of secondary MRM transitions of the primary MRM transition to the MRM cycle list and repeats steps (d)-(f).