发明名称 |
RECONSTITUTED NIPPLE SKIN MODEL |
摘要 |
A first subject of the invention is a skin model characterized in that it comprises keratinocytes capable of overexpressing filaggrin. A second subject of the invention is a method for evaluating the in vitro activity of a formulation or of at least one active agent on the healing of nipple skin and/or on the reduction of nipple skin inflammation, characterized in that said method comprises at least the following steps: a) bringing said active agent or said formulation into contact with a skin model comprising keratinocytes overexpressing filaggrin and also comprising a lesion; b) measuring the level of production of at least one biological marker in the nipple skin model of step a), characterized in that said biological marker can be chosen from lactate dehydrogenase (LDH), TNFα, filaggrin, TGFβ1 and β1 integrin; c) comparing the level of production of at least one biological marker obtained in step b) with a reference level of production; evaluating the efficacy of said active agent or of said formulation according to the comparison of step c). |
申请公布号 |
EP3092303(A1) |
申请公布日期 |
2016.11.16 |
申请号 |
EP20150700549 |
申请日期 |
2015.01.12 |
申请人 |
LABORATOIRES EXPANSCIENCE |
发明人 |
BREDIF, STÉPHANIE;BAUDOUIN, CAROLINE;MSIKA, PHILIPPE;MELONI, MARISA |
分类号 |
C12N5/071;C07K14/78;G01N33/50 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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