METHODS FOR NUCLEIC ACID AMPLIFICATION

摘要

The present disclosure provides novel methods for direct sample nucleic acid amplification with optional detection. The methods of the present disclosure provide for the foregoing without the requirement of nucleic acid purification from the sample. The methods generally comprise diluting a sample containing a nucleic acid target sequence to be amplified to produce a diluted sample, optionally subjecting the diluted sample to processing, either before or after dilution, and performing an amplification reaction on the sample to amplify the nucleic acid target sequence.

主权项

1. A method of amplifying a nucleic acid target sequence from a sample without purifying wthe nucleic acids from the sample, the method comprising:
(a) diluting the sample by at least 1:50; and (b) performing an amplification reaction on the sample to amplify the nucleic acid target sequence; wherein nucleic acids are not purified prior to performing either of steps (a) or (b).