发明名称 |
CAS9 CRYSTALS AND METHODS OF USE THEREOF |
摘要 |
The present disclosure provides atomic structures of Cas9 with and without polynucleotides bound thereto. Also provided is a computer-readable medium comprising atomic coordinates for Cas9 polypeptides in both an unbound configuration and a configuration wherein the Cas9 polypeptide is bound to one or more polynucleotides. The present disclosure provides crystals comprising Cas9 polypeptides; and compositions comprising the crystals. The present disclosure provides methods for the engineering of Cas9 polypeptides wherein Cas9 activity has been altered, ablated, or preserved and amended with additional activities. |
申请公布号 |
EP3089989(A1) |
申请公布日期 |
2016.11.09 |
申请号 |
EP20140876108 |
申请日期 |
2014.12.29 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
DOUDNA, JENNIFER A.;STERNBERG, SAMUEL H.;JINEK, MARTIN;JIANG, FUGUO;KAYA, EMINE;TAYLOR, DAVID |
分类号 |
C07K14/315;C12N9/00;C12P19/34 |
主分类号 |
C07K14/315 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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