MUTANT STAPHYLOCOCCUS BETA-GLUCURONIDASE ENZYMES WITH ENHANCED ENZYMATIC ACTIVITY

摘要

Mutated Staphylococcus sp. RLH1 β-glucuronidase enzymes with enhanced enzymatic activity and thermostability as compared to wild type enzyme are provided. The enzymes of the invention advantageously allow for accurate analysis of bodily samples for the presence of drugs in 30 minutes or less, as compared to the several hours needed using prior enzyme preparations. Methods of using the mutated enzymes for hydrolysis of glucuronide substrates, including opiates and benzodiazepines, are also provided.

主权项

1. A mutated Staphylococcus sp. RLH1 β-glucuronidase (StBGUS) enzyme comprising a substitution of an amino acid corresponding to G563 in SEQ ID NO: 42 with an amino acid comprising a side chain comprising a non-aromatic hydroxyl group or histidine or asparagine.