Serum-free Stable Transfection and Production of Recombinant Human Proteins in Human Cell Lines

摘要

The present invention relates to an improved method for the serum-free production of an immortalized human cell line stably transfected under serum-free conditions with a specific vector carrying the gene coding for the protein of interest. Furthermore the invention relates to a production cell line obtained by said method, a production method for said protein of interest utilizing said production cell line, and the specific vector carrying the gene of interest itself.

主权项

1. A method for recombinant production of a human target protein, the method comprising culturing a stably transfected immortalized human cell line comprising a nucleic acid sequence comprising a gene encoding a human target protein or a derivative or mutant thereof, a promoter, and a bovine growth hormone polyadenylation (polyA) signal, the promoter and polyA signal linked to the 5′ and 3′ end of the gene encoding the human target protein, respectively, under conditions sufficient for production of the human target protein, where the stably transfected immortalized human cell line is prepared by transfecting an immortalized human host cell line under serum-free conditions with a transfection vector comprising the nucleic acid sequence, to result in the stably transfected immortalized human cell line.