发明名称 |
NOVEL ELECTROCHEMICAL DNA BIOSENSOR USING GRAPHENE BIOCHIP FOR SPECIES IDENTIFICATION |
摘要 |
This study describes about a new electrochemical biosensor using DNA-redox electrostatic interaction and their subsequent non-specific adsorption on graphene screen-printed electrode or biochip. The ruthenium hexamine molecule [Ru(NH3)6]3+, or RuHex, was observed to form complexes with free DNA in solution that adsorbed onto graphene surfaces, enabling the development of a rapid, high-sensitivity DNA biosensor. Reproducible cathodic current signals were generated from these low-cost graphene biochips, both in the presence and absence of dsDNA and loop-mediated isothermal amplification (LAMP) amplicons. The combination of the DNA-redox molecule complexes and the graphene surface therefore provided a novel detection strategy. This new biosensor was able to identify different meat species based on the isothermal amplification of target genes followed by electrochemical detection with square wave voltammetry. |
申请公布号 |
US2016177387(A1) |
申请公布日期 |
2016.06.23 |
申请号 |
US201514968774 |
申请日期 |
2015.12.14 |
申请人 |
Roy Sharmili;Rahman Ibrahim Abdul;Ahmed Minhaz Uddin |
发明人 |
Roy Sharmili;Rahman Ibrahim Abdul;Ahmed Minhaz Uddin |
分类号 |
C12Q1/68;G01N27/30;G01N27/327;G01N33/12;G01N27/416 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. A biosensor, comprising:
a graphene surface; and a ruthenium hexamine molecule [Ru(NH3)6]3+ (RuHex), wherein the biosensor is used to detect a species in a food item. |
地址 |
GADONG BN |