METHOD FOR PROPAGATING STERILE MALE PLANT LINE

摘要

A method for propagating a sterile male plant line includes: (a) providing a first plant that comprises a homozygous recessive male sterility allele; (b) providing a second plant comprising the homozygous recessive allele and a construct, where the construct exists in a heterozygous state and comprises i) a first nucleotide sequence that will restore the male fertility of the first plant after expression, and ii) a second nucleotide sequence that is able to affect the grain shape or the endosperm nutrient material composition in a heterozygous state, which allows for distinguishing the grains with or without said construct by observation through naked eyes or devices; the first nucleotide sequence and the second nucleotide sequence are tightly connected with each other and coexist in plants; and (c) fertilizing the female gametes of the first plant with the male gametes of the second plant.

主权项

1. A method for maintaining a male sterile plant in a homozygous recessive state, the method comprising:
providing a first plant that comprises homozygous recessive male sterility alleles; providing a second plant, comprising homozygous recessive male sterility alleles the same as that in the first plant and a construct, wherein the construct exists in a heterozygous state and comprises:
i. a first nucleotide sequence that will restore the male fertility of the first plant after expression in the first plant;ii. a second nucleotide sequence that is able to affect grain shape or endosperm nutrient material composition in a heterozygous state, which allows for distinguishing the grains with or without the construct by observation through naked eyes or devices; wherein the first nucleotide sequence and the second nucleotide sequence are tightly connected with each other and coexist in a plant; fertilizing female gametes of the first plant with male gametes of the second plant to produce progenies maintaining the homozygous recessive state of the first plant.