DESIGN AND CONSTRUCTION OF DIVERSE SYNTHETIC PEPTIDE AND POLYPEPTIDE LIBRARIES

摘要

The present invention concerns the design and construction of diverse peptide and polypeptide libraries. In particular, the invention concerns methods of analytical database design for creating datasets using multiple relevant parameters as filters, and methods for generating sequence diversity by directed multisyntheses oligonucleotide synthesis. The present methods enable the reduction of large complex annotated databases to simpler datasets of related sequences, based upon relevant single or multiple key parameters that can be individually directly defined. The methods further enable the creation of diverse libraries based on this approach, using multisynthetic collections of discrete and degenerate oligonucleotides to capture the diverse collection of sequences, or portions thereof.

主权项

1. A method for diversity analysis of a database comprising related amino acid sequences characterized by at least one shared sequence motif, comprising the steps of:
(a) aligning said related amino acid sequences; (b) creating a first dataset by applying a predetermined combination of two or more filters to said related amino acid sequences comprising said shared sequence motif; (c) analyzing said first dataset for positional amino acid usage frequency within said shared sequence motif; and (d) creating a second dataset characterized by a minimum threshold amino acid usage frequency at one or more amino acid positions within said shared sequence motif.