Sorghum Centromere Sequences and Minichromosomes

摘要

The invention is generally related to MCs containing sorghum centromere sequences. In addition, the invention provides for methods of generating plants transformed with these MCs. MCs with novel compositions and structures are used to transform plants cells which are in turn used to generate the plant. Methods for generating the plant include methods for delivering the MC into plant cell to transform the cell, methods for selecting the transformed cell, and methods for isolating plants transformed with the MC.

主权项

1. A sorghum plant comprising a sorghum mini-chromosome comprising a sorghum centromere, wherein the sorghum centromere comprises at least two copies of a first repeated nucleotide sequence that is at least 80% identical to the nucleotide sequence of any one of SEQ ID NOs:22-176 or hybridizes to the nucleotide sequence of any one of SEQ ID NOs:22-176 under stringent conditions comprising hybridization at 65° C. and washing three times for 15 minutes with 0.25×SSC, 0.1% SDS at 65° C.