| 发明名称 |
Live bacterial vaccines for prophylaxis or treatment of infection |
| 摘要 |
A live bacterium, having a DNA construct stabilized against transduction of other bacteria, having a promoter sequence and encoding a fusion peptide, comprising a bacterial secretion peptide portion and a non-bacterial immunogenic polypeptide portion, having a nucleotide sequence coding for the non-bacterial immunogenic polypeptide portion which has at least one codon optimized for bacterial expression. The bacterium has a secretion mechanism which interacts with at least the bacterial secretion peptide portion to cause a secretion of the fusion peptide from the bacterium, and a genetic virulence attenuating mutation. The bacterium is adapted to act as an animal vaccine, to transiently infect a tissue of the animal, and cause an immunity response to the non-bacterial immunogenic polypeptide portion in the animal to a non-bacterial organism associated with the non-bacterial immunogenic polypeptide portion. |
| 申请公布号 |
US9315817(B2) |
申请公布日期 |
2016.04.19 |
| 申请号 |
US201313892380 |
申请日期 |
2013.05.13 |
| 申请人 |
|
发明人 |
Bermudes David Gordon |
| 分类号 |
C12P1/00;C12P21/06;C12N1/00;C12N1/20;C12N15/74;A61K39/145;C12N1/36;C12N9/24;A61K39/12;A61K39/00 |
主分类号 |
C12P1/00 |
| 代理机构 |
Ostrolenk Faber LLP |
代理人 |
Hoffberg, Esq. Steven M.;Ostrolenk Faber LLP |
| 主权项 |
1. A live bacterium, comprising:
a stabilized chromosomally integrated DNA construct having a prokaryotic promoter sequence and encoding a fusion peptide, said fusion peptide comprising a bacterial secretion peptide portion and a heterologous immunogenic polypeptide portion, wherein the chromosomally integrated DNA construct has a nucleotide sequence coding for the heterologous immunogenic polypeptide portion, wherein the chromosomally integrated DNA construct is stabilized with respect to a wild type bacterium of the same strain against transduction of other bacteria; and said bacterium having a secretion mechanism which interacts with at least the bacterial secretion peptide portion to cause a secretion of the fusion peptide from the bacterium; the bacterium having a genetic virulence attenuating mutation and being adapted to act as an animal vaccine, to transiently colonize a solid tissue organ of the animal in a non-lethal manner, and cause an immune response to the heterologous immunogenic polypeptide portion to cause an immunity in the animal to an organism associated with the heterologous immunogenic polypeptide portion. |
| 地址 |
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