| 摘要 |
Some aspects of this disclosure relate to the identification of reactive nucleic acids, such as, for example, reactive cellular RNAs. Minimal reactive motif consensus sequences and isolated reactive nucleic acids conforming to such consensus sequences are provided herein. Reactive probes that selectively form covalent bonds with reactive nucleic acids are also provided herein, as are methods of using such probes, for example, for labeling, identification, characterization, and/or quantification of reactive nucleic acids. This disclosure further provides fusion molecules comprising a reactive nucleic acid conjugated to a heterologous molecule of interest, such as a heterologous nucleic acid or protein. Methods and kits for generating and using such fusion proteins to track, detect, and/or quantify molecules of interest in a biological sample are also provided as are expression constructs encoding fusion molecules of reactive nucleic acids and heterologous nucleic acids of interest. |
