发明名称 |
RIBONUCLEOTIDE TAG NUCLEIC ACID DETECTION |
摘要 |
The present application provides polynucleotides comprising 5'-tails with sequence segments useful for the detection of target nucleic acid sequences, and methods for their use in detecting target nucleic acids. The polynucleotides are used to amplify a subsequence of a target nucleic acid in the presence of one or more ribonucleotides. The ribonucleotides are incorporated into amplification products at regular intervals complementary to the 5'-tail sequence segments. Cleavage of amplification products at the bond immediately 3' to incorporated ribonucleotides produces detectably distinct fragments indicative of the presence or absence of a target nucleic acid. |
申请公布号 |
HK1141559(A1) |
申请公布日期 |
2016.03.24 |
申请号 |
HK20100107755 |
申请日期 |
2010.08.13 |
申请人 |
COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES A |
发明人 |
KEITH A. BAUER;DAVID H. GELFAND;IVO GLYNNE GUT;FLORENCE MAUGER |
分类号 |
C12Q;C12M;C12N |
主分类号 |
C12Q |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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