HERPES SIMPLEX VIRUS (HSV) WITH MODIFIED TROPISM, USES AND PROCESS OF PREPARATION THEREOF

摘要

A modified Herpes Simplex Virus (HSV), which has a portion of gD (glycoprotein D) of the glycoproteic envelope deleted and a heterologous single chain antibody inserted in place of such deleted portion; the modified HSV is capable of infecting cells through receptor HER2/ErbB2 but not through receptors HVEM/HveA and nectin1/HveC; uses of the modified HSV and a process of the preparation thereof are also disclosed.

主权项

1. A modified herpes simplex virus (HSV) comprising a glycoproteic envelope, which has an heterologous peptide ligand capable of specifically binding to a given receptor expressed by diseased cells and substantially not expressed by non-diseased cells; the glycoproteic envelope being so modified that the capability of the modified HSV of interacting with receptor nectin1/HveC is reduced, wherein the peptide ligand replaces a deleted portion of glycoprotein D (gD) between residues 1 to 8 and 38 to 55 of gD, or between residues 40 to 61 and 210 to 218 of gD, wherein said residue numbers correspond to the residue numbering of SEQ ID NO: 1.