发明名称 |
EXOSOME ISOLATION |
摘要 |
A method of isolating exosomes from a biological sample is provided. The method includes the steps of: i) exposing the biological sample to a first centrifugation to remove cellular debris greater than about 7-10 microns in size from the sample and obtaining the supernatant following centrifugation; ii) subjecting the supernatant from step i) to centrifugation to remove microvesicles therefrom; iii) microfiltering the supernatant from step ii) and collecting the microfiltered supernatant; iv) subjecting the microfiltered supernatant from step iii) to at least one round of ultracentrifugation to obtain an exosome pellet; and v) re-suspending the exosome pellet from step iv) in a physiological solution and conducting a second ultracentrifugation in a density gradient and remove the exosome pellet fraction therefrom. The method advantageously results in exosomes that maintain integrity, stability and are essentially free from contaminating particles. |
申请公布号 |
WO2016033695(A1) |
申请公布日期 |
2016.03.10 |
申请号 |
WO2015CA50853 |
申请日期 |
2015.09.04 |
申请人 |
EXERKINE CORPORATION |
发明人 |
TARNOPOLSKY, MARK;SAFDAR, ADEEL |
分类号 |
C12N5/071;A61K9/48;A61K35/12;C07H21/00;C07K17/02;C12N5/07;C12N15/10;C12N15/11 |
主分类号 |
C12N5/071 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|