| 摘要 |
The invention described herein relates to methods of screening for pro-inflammatory genes and anti-inflammatory genes which may be useful for treating an inflammatory disease, disorder, or otherwise abnormal condition, such as an inflammatory lung disease. The identified pro-inflammatory genes and anti-inflammatory genes may be used to produce pharmaceutical compositions for use in treating the inflammatory disease, disorder, or otherwise abnormal condition. |
| 主权项 |
1. A method of identifying a target gene which may be useful for treating an inflammatory disease, the method comprising:
a) providing a population of test cells, wherein:
1) the test cells are a clonal expansion of a single epithelial stem cell capable of propagating for at least about 20 (30, 40, 50, 60, 70, 100, 150, 200, 250, 300, 350, 400 or more) doublings while maintaining a multipotent phenotype; or,2) the test cells are differentiated from said clonal expansion of said single epithelial stem cell; b) contacting the test cells with a pro-inflammatory cytokine associated with the inflammatory disease; c) identifying one or more genes the expression level of which has been modulated upon contacting the pro-inflammatory cytokine, as compared to that of control test cells not contacted by the pro-inflammatory cytokine, wherein said one or more genes identified in step c) are target gene(s) which may be useful for treating the inflammatory disease. |
