Detection of RNA-interacting regions in DNA

摘要

The present invention provides methods and kits for detecting RNA-interacting regions in genomic DNA.

主权项

1. A method of detecting RNA-interacting regions in genomic DNA, the method comprising:
introducing an RNA-degrading agent and a DNA-degrading agent into a nucleus, whereby at least one DNA region in the genomic DNA is degraded by the DNA-degrading agent due to the presence of the RNA-degrading agent; detecting the at least one DNA region in the genomic DNA that is degraded by the DNA-degrading agent; and comparing the amount of the at least one DNA region that is detected to a control sample in which the genomic DNA is contacted by the DNA-degrading agent but not the RNA-degrading agent, wherein an absence or reduction in the quantity of copies of a DNA region, relative to the control sample, indicates that the DNA region is degraded by the DNA-degrading agent; thereby detecting the RNA-interacting regions.