Method of producing a sweet protein

摘要

The present invention relates to a method of producing a recombinant sweet protein in a filamentous fungus. The invention also relates to isolated polynucleotides encoding the sweet protein and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, as well as methods of using the sweet protein produced by the method of the invention.

主权项

1. A method of producing a recombinant sweet protein comprising an amino acid sequence having at least 90% identity to amino acids 2 to 54 of SEQ ID NO: 2, which comprises:
a) cultivating an Aspergillus host cell comprising a nucleic acid construct comprising a polynucleotide encoding the sweet protein under conditions conducive for production of the sweet protein; and b) recovering the sweet protein wherein the sweet protein is secreted from the host cell in a functional form.