Systems And Methods For Baseline Correction Using Non-Linear Normalization

摘要

Systems and methods are provided for calibrating emission data or other information signals collected during a polymerase chain reaction (PCR), amplification reaction, assay, process, or other reaction. Calibration of multiple detectable materials can be achieved during a single cycle or run, or during a plurality of runs of the reaction. A reading from every well, container, or other support region of a sample support does not have to be taken. Interpolation can be used to determine values for emission data or other information signals that were not taken, or are unknown, using detected emission data, or other detected information signals. By calibrating the detected emission data and the interpolated data, a more accurate reading of emission data or information signal can be obtained.

主权项

1. A computer-implemented method for generating a normalized amplification profile, the method comprising:
receiving emission data detected in an amplification reaction; identifying a baseline signal in the emission data; generating a normalization quantity based on the emission data and the identified baseline signal; generating the normalized amplification profile based on a ratio of the emission data to the normalization quantity; generating a threshold cycle based on the normalized amplification profile; and quantifying an original sample amount based on the threshold cycle.