Fermentation processes for cultivating <i>Streptococci </i>and purification processes for obtaining cps therefrom

摘要

This invention is in the field of bacterial cultures and specifically relates to the optimization of culture conditions to improve the production of bacterial capsular polysaccharides from Streptococcus strains in fed batch culture and to novel purification methods suitable for production scale purification of bacterial capsular polysaccharides from Streptococcus strains resulting in higher levels of purity than previously obtained for production scale.

主权项

1. A fed batch method for cultivating Streptococcus for production of capsular polysaccharides (cps), wherein said fed batch method comprises (a) providing an inoculum of a strain of Streptococcus expressing the cps, and (b) cultivating the strain by fermentation, wherein said cultivating comprises a pH-independent linear addition of a carbon source to a cultivating medium.