Fractionation of Oil-Bearing Microbial Biomass

摘要

The invention generally relates to the production of hydrocarbon compositions, such as a lipid, in microorganisms. In particular, the invention provides methods for extracting, recovering, isolating and obtaining a lipid from a microorganism and compositions comprising the lipid. The invention also discloses methods for producing hydrocarbon compositions for use as biodiesel, renewable diesel, jet fuel, and other materials.

主权项

1. A method of extracting a lipid from a recombinant microalga, the method comprising the steps of:
(a) lysing recombinant microalgal cells to produce a lysate, wherein the recombinant microalgal cells:
(i) have not been subjected to a drying step between culturing and lysing; and(ii) contain a lipid; (b) treating the lysate with an organic solvent for a period of time, wherein the organic solvent is an alcohol selected from methanol, ethanol, isopropanol or butanol; and (c) separating the treated lysate into layers comprising a lipid layer and an aqueous layer and, optionally, a lipid:aqueous emulsion layer, and/or a cell pellet; and (d) removing the lipid from the other layer(s).