Recombinant phage and methods

摘要

This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.

主权项

1. A method of making a recombinant phage genome, comprising:
(a) selecting a starting phage genome and a eukaryotic vector; (b) selecting a stitching oligonucleotide or oligonucleotide duplex for recombination with the starting phage genome and the eukaryotic vector comprising:
(i) detecting an imperfect oligo repeat at an end of the starting phage genome, and selecting a stitching oligonucleotide comprising a sequence from the ends of the starting phage genome and a sequence flanking the double strand break in the eukaryotic vector;(ii) detecting a perfect oligo repeat at an end of the starting phage genome and selecting a oligonucleotide duplex comprising a sequence homologous to the starting phage genome and a sequence homologous to the eukaryotic vector; (c) introducing the starting phage genome and the eukaryotic vector of step (a), and the stitching oligonucleotide or the oligonucleotide duplex of step (b) into a plurality of eukaryotic vector host cells that are not phage host cells; (d) selecting a vector host cell comprising a recombinant vector comprising an intact starting phage genome as a result of insertion of the starting phage genome into the vector; and (e) inserting a heterologous nucleic acid sequence comprising an open reading frame encoding a screenable marker into the intact starting phage genome to provide a recombinant intact phage genome, wherein the screenable marker is operatively linked to a regulatory control sequence.