| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a porous cell scaffold of a 2 mm or more thickness and a production method thereof, easily seeding cells in its scaffold without applying any large force to the cells, and substantially accurately obtaining the amount of cells in the scaffold as well in seeding. <P>SOLUTION: After filling a guiding solution with kinematic viscosity being 50-450% of that of a culture medium, in a whole continuous small hole structure having hole diameters of 5-3,200 μm and an average hole diameter of 50-1,500 μm, of a sheet-shaped or block-shaped scaffold having a thickness of 2 mm or more, a culture medium with cells being suspended to an upper side of the scaffold is supplied, and the guiding solution from a lower side of the scaffold is sucked by low suction force to thereby cause the culture medium with cells being suspended to enter the whole small hole structure. A water absorber such as a filter paper is preferably used for sucking the guiding solution by low suction force. <P>COPYRIGHT: (C)2009,JPO&INPIT |
