| 摘要 |
<p>Provided is a microscope capable of efficiently performing 3D imaging irrespective of the shape of a specimen. Employed is a laser microscope including: an area segmenting section (31) that segments an observation range of a specimen in the directions perpendicular to the optical axis of an objective lens into a plurality of areas; a surface-position storing section (32) that stores positions of an motorized stage in association with surface positions of the specimen, for the plurality of areas; a surface-shape estimating section (34) that estimates the surface shape of the specimen from the positions of the motorized stage and the surface positions of the specimen stored in the surface-position storing section (32) for the plurality of areas; a Z-scanning condition determining section (35) that determines surface positions of the specimen at desired positions of the motorized stage from the surface shape of the specimen estimated by the surface-shape estimating section (34); and a light detecting section that detects light from the specimen over certain ranges specified with reference to the surface positions of the specimen determined by the Z-scanning condition determining section (35), in the optical axis direction of the objective lens.</p> |
