发明名称 |
PRODUCTION OF RED BLOOD CELLS AND PLATELETS FROM STEM CELLS |
摘要 |
This disclosure provides methods of making a megakaryocyte-erythroid progenitor cell (MEP), comprising differentiating a stem cell into a MEP in culture in the presence of an aryl hydrocarbon receptor (AhR) agonist. In some embodiments the stem cell is a pluripotent stem cell. In some embodiments the MEP co-expresses CD41 and CD235. In some embodiments the number of MEPs produced in the culture increases exponentially. Methods of making a red blood cell (RBC) by culturing a MEP in the presence of an AhR agonist are also provided. Methods of making a megakaryocyte and/or a platelet, comprising culturing a MEP in the presence of an AhR modulator are also provided. In some embodiments the AhR modulator is an AhR antagonist. This disclosure also provides compositions comprising at least 1 million MEPs per ml and compositions in which at least 50% of the cells are MEPs. |
申请公布号 |
US2015335682(A1) |
申请公布日期 |
2015.11.26 |
申请号 |
US201514729008 |
申请日期 |
2015.06.02 |
申请人 |
Boston Medical Center Corporation ;Trustees of Boston University |
发明人 |
Murphy George J.;Sherr David H.;Rozelle Sarah S.;Smith Brenden W. |
分类号 |
A61K35/19;G01N33/50;A61K45/06;C12N5/078 |
主分类号 |
A61K35/19 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Boston MA US |