发明名称 |
Fungal production system |
摘要 |
The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous β-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided. |
申请公布号 |
US9175296(B2) |
申请公布日期 |
2015.11.03 |
申请号 |
US201013138661 |
申请日期 |
2010.03.16 |
申请人 |
DYADIC NEDERLAND B.V. |
发明人 |
Punt Peter J.;Burlingame Richard Paul;Pynnonen Christine M.;Olson Phillip T.;Wery Jan;Visser Johannes Heinrich;Emalfarb Mark A.;Visser Jacob;Verdoes Jan Cornelis |
分类号 |
C12P21/00;C12N15/80;C12N1/14;C12N9/12;C12N9/34;C12N15/01;C12P21/02;C12R1/645;C12N9/42;C12N9/24 |
主分类号 |
C12P21/00 |
代理机构 |
Browdy and Neimark, PLLC |
代理人 |
Browdy and Neimark, PLLC |
主权项 |
1. A strain of Chrysosporium lucknowense selected from the group consisting of W1L deposited at CBS under accession number 122189, W1L#100.1 deposited at CBS under accession number 122190, and a strain derived therefrom by gene disruption. |
地址 |
Wageningen NL |