| 摘要 |
<p>The invention relates to an analytical immunosensor device and to a method for the constructing same, based on the sequential deposition of self-assembled monolayers of polymers and affinity elements, applicable for the detection and quantification of any target antigen or analyte in a liquid sample, and allowing the implementation of the "quasi-reagentless" or "reagentless" displacement assay, the minimisation of the non-specific adsorption and, as a result, the reduction of the detection limit. Said device is designed on the basis of a sensor surface covered with polymer monolayers (redox or non-redox) on which are deposited sub-monolayers of affinity elements, in order to subsequently carry out the affinity reaction with an antigen, pseudo-antigen or a hapten marked with a protein, preferably an enzyme (redox or non-redox) or a nanoparticle, allowing said method for constructing the device to develop "a la carte" immunosensors, with an electrochemical, optical and/or piezoelectric transduction of the signal.</p> |
