Electrode, electrochemical sensor and apparatus, and methods for operating the same

摘要

A method for amperometric detection of proteins, especially haemoglobin in faeces, using an electrochemical sensor. The electrochemical sensor includes: a working electrode having an electrically conductive matrix holding a first reagent and/or a second reagent, the second reagent being an oxidizing agent, or a precursor thereof, for the first reagent; a counter electrode and optionally a reference electrode; wherein a reaction between the first reagent and the oxidizing agent is catalyzed by the protein to provide a detectable signal at the working electrode. The electrically conductive matrix is an electrically conductive carbon- or graphite-containing matrix or an electrically conductive porous matrix.

主权项

1. A method for detecting a protein, the method comprising the steps of:
(i) providing an electrochemical sensor, the electrochemical sensor comprising:
a working electrode comprising an electrically conductive matrix holding a first reagent and/or a second reagent, the second reagent being an oxidising agent or a precursor thereof for the first reagent;a counter electrode and optionally a reference electrode;wherein a reaction between the first reagent and the oxidising agent is catalysable by the protein to provide a detectable signal at the working electrode; andthe electrically conductive matrix is an electrically conductive carbon- or graphite containing matrix or an electrically conductive porous matrix; (ii) making available the electrochemical sensor at an environment to be analysed for the protein; (iii) making available for reaction the first reagent and the second reagent; (iv) maintaining a potential across the working electrode and the counter electrode and/or the reference electrode, where present; and (v) measuring the current passing between the working electrode and the counter and/or reference electrode where present.