IMPROVED CULTIVATION MEDIA AND PROCESS FOR IMPROVED PROTEIN PRODUCTION BY PICHIA STRAINS

摘要

The present invention provides optimized cell culture media and fed-batch cultivation processes to improve the viability and volumetric production of heterologous proteins in Pichia. The disclosed media and processes utilize a non-fermentable sugar or sugar alcohol as an osmoprotectant to improve the robustness of Pichia production strains during methanol inducible fermentation.