METHOD AND KIT

摘要

The present invention provides a kit comprising: a first primer set comprising at least two primers between 10 and 60 nucleotides in length, wherein the primers are capable of hybridizing under very high stringency conditions to a M. tuberculosis complex nucleic acid sequence shown in SEQ ID NO: 1, and wherein the primers are capable of amplifying the M. tuberculosis complex nucleic acid; and/or a second primer set comprising at least two primers between 10 and 60 nucleotides in length, wherein the primers are capable of hybridizing under very high stringency conditions to a Legionellaceae nucleic acid sequence shown in SEQ ID NO: 9, and wherein the primers are capable of amplifying the Legionellaceae nucleic acid; and/or a third primer set comprising at least two primers between 10 and 60 nucleotides in length, wherein the primers are capable of hybridizing under very high stringency conditions to a M. pneumoniae nucleic acid sequence shown in SEQ ID NO: 20, and wherein the primers are capable of amplifying the M. pneumoniae nucleic acid. The invention also provides a method for detecting the presence of one or more of Mycobacterium tuberculosis complex, Legionellaceae and Mycoplasma pneumoniae in a sample, comprising:_a. amplifying one or more of a M. tuberculosis complex nucleic acid, a Legionellaceae nucleic acid and a M. pneumoniae nucleic acid in a sample, wherein amplifying the M. tuberculosis complex nucleic acid comprises contacting the sample with at least one first primer between 10 and 60 nucleotides in length, wherein the primer is capable of hybridizing under very high stringency conditions to a M. tuberculosis complex nucleic acid sequence shown in SEQ ID NO: 1, and wherein the primer is capable of amplifying the M. tuberculosis complex nucleic acid; wherein amplifying the Legionellaceae nucleic acid comprises contacting the sample with at least one second primer between 10 and 60 nucleotides in length, wherein the primer is capable of hybridizing under very high stringency conditions to a Legionellaceae nucleic acid sequence shown in SEQ ID NO: 9, and wherein the primer is capable of amplifying the Legionellaceae nucleic acid; and wherein amplifying the M. pneumoniae nucleic acid comprises contacting the sample with at least one third primer between 10 and 60 nucleotides in length, wherein the primer is capable of hybridizing under very high stringency conditions to a M. pneumoniae nucleic acid sequence shown in SEQ ID NO: 20, and wherein the primer is capable of amplifying the M. pneumoniae nucleic acid; and b. detecting amplification of one or more of a Mycobacterium tuberculosis complex nucleic acid, a Legionellaceae nucleic acid and a Mycoplasma pneumoniae nucleic acid if present in the sample.