MICROFLUIDIC METHOD AND SYSTEM FOR ENZYME INHIBITION ACTIVITY SCREENING

摘要

Methods for screening a compound for enzyme inhibition activity include providing at least one sample mixture to a microfluidic device, applying vacuum pressure to the sample mixture, flowing the sample mixture along a microchannel of the microfluidic device, separating at least two components of the sample mixture based upon a net charge difference between the product and at least one other material to produce separated material, detecting at least one of the separated materials, and determining enzyme inhibition activity based on the detection of the separated material. Kits for screening a compound for enzyme inhibition activity include a first multiwell plate 150 having a specific plurality of enzymes disposed within a first plurality of wells and a second multiwell plate 160 having a plurality of enzyme substrates disposed with a second plurality of wells, a phosphate source 162 and a cofactor 164 disposed within each well of the second plate.