发明名称 |
METHODS FOR PRODUCING SUBSTANTIALLY HOMOGENEOUS HYBRID OR COMPLEX N-GLYCANS IN METHYLOTROPHIC YEASTS |
摘要 |
The present invention provides methods for effectively and efficiently converting methylotrophic yeast's heterogeneous high mannose-type N-glycosylation to mammalian-type N-glycosylation by disruption of an endogenous glycosyltransferase gene (OCH1) and step-wise introduction of heterologous glycosidase and glycosyltransferase activities. Each engineering step includes a number of stages: transformation with an appropriate vector, cultivation of a number of transformants, performance of sugar analysis and heterologous protein expression analysis, and selection of a desirable clone. The selected clone is then subjected to the next engineering step. |
申请公布号 |
US2012029174(A1) |
申请公布日期 |
2012.02.02 |
申请号 |
US201113241543 |
申请日期 |
2011.09.23 |
申请人 |
CALLEWAERT NICO;WIERSMA DAVID A.;VIB, VZW;RESEARCH CORPORATION TECHNOLOGIES, INC.;UNIVERSITEIT GENT |
发明人 |
CALLEWAERT NICO;WIERSMA DAVID A. |
分类号 |
C07K14/39;C12P21/00 |
主分类号 |
C07K14/39 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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