| 摘要 |
<p>Sample Slides for Laser Desorption Ionisation (LDI) Mass Spectrometry and Optical Microscopy Indium tin oxide coated slides, their synthesis and use in imaging mass spectrometry are provided in the present application. The methods of synthesis described herein may be used to prepare indium tin oxide thin films on glass substrates with the required nanometric surface structure for use in the matrix-free imaging of metabolites and small-molecule drugs, without affecting the transparency required for optical microscopy. The custom-made slides were tested as potential substrates for imaging mass spectrometry by measuring a range of different properties, including elemental composition, topology, optical and electrical properties and hydrophobicity and comparing them with commercially available slides. In a preferred embodiment, the sample slide (ITO4) has an indium tin oxide surface layer between 25-35 nm in thickness, a surface roughness of about 1.2 nm, a resistivity of between 1.5-1.9 microOhrn/cm2 and an optical transmission at 355nm of greater than 60%.</p> |
