Insect resistant plant

摘要

The present invention relates to novel pepper plants resistant to insects, and to seeds and fruits of said plants. The present invention also relates to methods of making and using such plants and their fruits. The invention further relates to markers and the use thereof in marker assisted breeding and for identifying the insect resistance trait. In particular, the present invention provides a cultivated Capsicum annuum plant which is resistant, particularly intermediately resistant, to infestations by insects of the family Thripidae and/or the genus Bemisia, but especially to infestations by Bemisia tabaci and Frankliniella occidentalis.

主权项

1. A method for introducing at least one allele associated with resistance to Bemisia at a quantitative trait locus (“QTL”) contributing to resistance to Bemisia into a Capsicum annuum plant lacking said allele comprising:
a) obtaining a first Capsicum annuum plant wherein said plant contains a genome comprising at least one QTL located on chromosome 3 or chromosome 5 which contributes to Bemisia resistance; b) crossing said first Capsicum annuum plant with a second Capsicum annuum plant, wherein said second Capsicum annuum plant lacks said QTL; and c) identifying a C. annuum plant resulting from the cross exhibiting increased resistance to Bemisia comprising detecting, by using at least one oligonucleotide primer or primer pair, at least one marker allele located on chromosome 3 or chromosome 5 and co-segregating with said Bemisia resistance,wherein said at least one marker allele co-segregating with said Bemisia resistance is characterized by the PCR amplification product of
i. a PCR primer or primer pair selected from the group consisting of primer pairs 1-6 represented by forward and reverse primers of SEQ ID NOs: 1-12, including primer pairs resulting from a combination of the forward and reverse primers of SEQ ID NOs: 1-12; and/or ii. a PCR primer pair selected from the group consisting of primer pairs 7-13 represented by forward and reverse primers of SEQ ID NOs: 13-26, including primer pairs resulting from a combination of the forward and reverse primers of SEQ ID NOs: 13-26.