| 摘要 |
Avian, diploid cell lines, EBx, that are non-tumorigenic, immortal and non-transformed are new. Avian, diploid cell lines, EBx, that are non-tumorigenic, immortal and non-transformed are new. They are characterized by: the round, compact morphology of avian embryonal stem cells with high nucleus:cytoplasm ratio; population doubling time 15-30, preferably 16-24, hours; endogenous alkaline phosphatase and telomerase activities; and reaction with antibodies specific for: (i) the markers SSEA-1 (TEC01) and EMA-1 and (ii) cell-surface receptors that contain the oligosaccharides SAalpha 2-3 and SAalpha 2-6. Independent claims are also included for the following: (1) method for producing influenza virus (IV) by replication in EBx; (2) method for producing a viral vaccine from IV prepared by method (1); (3) method for preparing antigenic surface proteins (A) from intact IV, prepared by method (1); and (4) vaccines against influenza, killed or live, prepared either by method (2) or from (A). ACTIVITY : Virucide. No details of tests for virucidal activity are given. MECHANISM OF ACTION : Vaccine. |
