Method of Producing a Normalised Nucleic Acid Library Using Solid State Capture Material

摘要

The present invention provides a method of preparing a nucleic acid library, which includes providing a one or more nucleic acid samples, and a one or more of samples of solid state capture material; contacting each nucleic acid sample with a sample of capture material to provide captured nucleic acid samples; and pooling the captured nucleic acid samples to provide the nucleic acid library. The method is particularly suitable for preparing nucleic acids for sequencing, especially next generation sequencing and related methods such as genotyping-by-sequencing.

主权项

1. A method of preparing a normalised nucleic acid library for containing a plurality of nucleic acid species in pre-defined relative concentrations, said method including:
providing
a plurality of samples each containing a biotinylated nucleic acid species, anda solid state capture material including streptavidin; contacting each of the plurality of nucleic acid-containing samples with a limiting amount of the solid state capture material to provide capture some but not all of the nucleic acids from each of the nucleic-acid containing samples; and pooling the captured nucleic acid samples, and optionally eluting the captured nucleic acids from the solid state capture material to provide the normalised nucleic acid library containing the plurality of nucleic acid species in relative concentrations determined by the amount of solid state capture material contacted with the sample.