发明名称 |
HIGH-THROUGHPUT GENOTYPING BY SEQUENCING LOW AMOUNTS OF GENETIC MATERIAL |
摘要 |
The present invention provides a method for analysis of target nucleic acids which are present in low amounts. In particular, the method comprises the following steps: i. providing a sample wherein target nucleic acids are present in a low amount, ii. generating a reduced representation library of said target nucleic acids by a method comprising: fragmenting said target nucleic acids; ligating adaptors to said fragments; and selecting a subset of said adaptor-ligated fragments, iii. massively parallel sequencing said reduced representation library, and iv. identifying variants in said target nucleic acids by analyzing results obtained by said sequencing. |
申请公布号 |
US2015247184(A1) |
申请公布日期 |
2015.09.03 |
申请号 |
US201314430293 |
申请日期 |
2013.10.07 |
申请人 |
KATHOLIEKE UNIVERSITEIT LEUVEN, KU LEUVEN R&D |
发明人 |
Vermeesch Joris;Voet Thierry;Hannes Femke;Van Houdt Jeroen;Maes Gregory |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. A method for analysis of target nucleic acids, the method comprising:
i. providing a sample wherein target nucleic acids are present in a low amount, ii. generating a reduced representation library of said target nucleic acids by a method comprising:
fragmenting said target nucleic acids;ligating adaptors to said fragments; andselecting a subset of said adaptor-ligated fragments, iii. massively parallel sequencing said reduced representation library, and iv. identifying variants in said target nucleic acids by analyzing results obtained by said sequencing. |
地址 |
Leuven BE |