Codon optimized sodium-iodide symporter gene and its use

摘要

The present disclosure provides a polynucleotide which is codon optimized for the efficient expression in a eukaryotic cell, a plasmid and a eukaryotic cell comprising the same. The modification resulted in the efficient expression of NIS in eukaryotic cells and the enhancement of the function of NIS by glycosylation. Thus modified polynucleotide encoding NIS of the present disclosure is useful as imaging reporter for gene, viral and/or cell based therapies.

主权项

1. A polynucleotide encoding a sodium-iodide symporter (NIS) protein having the sequence set forth in SEQ ID NO: 2 and codon optimized for expression in a eukaryotic cell.