Apparatus for delivery of laser-beams of different wavelengths to a flow-cytometer

摘要

An optical apparatus for delivering to a flow-cell of a flow-cytometer a plurality of beams of laser radiation each thereof having a different wavelength. The apparatus includes a dispersion compensation-prism and a plurality of directing-prisms equal in number to the number of laser-beam. The directing-prisms are arranged to direct the laser radiation beams directly therethrough into the dispersion compensation-prism as converging fan of beams in a first plane. The beams are transmitted by the compensation-prism as a converging fan of beams intersecting then proceeding as a diverging fan of beams in the first plane. A spherical focusing lens is arranged cooperative with a cylindrical lens for focusing the plurality of laser-beams as a plurality of spaced apart elongated focal spots in a plane in which the cytometer flow-cell is located.

主权项

1. Optical apparatus for delivering to a flow-cell of a flow-cytometer a plurality of beams of laser radiation, each thereof having a different wavelength, the apparatus comprising:
a dispersion-compensation prism; a plurality of directing-prisms equal in number to the number of laser-beams in the plurality thereof, the directing-prisms arranged to transmit the laser radiation beams directly therethrough, without internal reflection, and direct the laser-radiation beams into the dispersion compensation-prism, as a converging fan of beams in a first plane, with the beams transmitted by the dispersion-compensation prism as a converging fan of beams intersecting then proceeding as a diverging fan of beams in the first plane; and a plurality of optical elements including a cylindrical optical element, the plurality of optical elements configured and arranged to focus the diverging fan of laser-radiation beams as a plurality of spaced-apart elongated focal spots at a location in which the cytometer flow-cell is positioned.