Yeast Cells Having Reductive TCA Pathway from Pyruvate to Succinate and Overexpressing an Exongenous NAD(P)+ Transhydrogenase Enzyme

摘要

Yeast cells having a reductive TCA pathway from pyruvate or phosphoenolpyruvate to succinate, and which include at least one exogenous gene overexpressing an enzyme in that pathway, further contain an exogenous transhydrogenase gene.

主权项

1. A recombinant yeast cell which has an active reductive TCA pathway from pyruvate or phosphoenolpyruvate to succinate, further metabolizes succinate to one or more succinate metabolization products and overexpresses a NAD(P)+ transhydrogenase enzyme and which further has integrated into its genome one or more of (i) an exogenous pyruvate carboxylase gene that encodes for an enzyme which catalyzes the conversion of pyruvate to oxaloacetate, (ii) an exogenous malate dehydrogenase gene which encodes for an enzyme that catalyzes the conversion of oxaloacetate to malate (iii) an exogenous fumarase gene that encodes for an enzyme which catalyzes the conversion of malate to fumarate and (iv) an exogenous fumarate reductase gene which encodes for an enzyme which catalyzes the conversion of fumarate to succinate.