Mutant β-glucosidase, enzyme composition for decomposing biomass, and method of producing sugar solution

摘要

A β-glucosidase exhibits high activity in the presence of biomass and has high thermal stability compared to conventional enzymes. The β-glucosidase includes substitutions and/or deletions of amino acids, at least three amino acids selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227, and Glu330, of the parent β-glucosidase with other amino acids and exhibits a decomposition activity.

主权项

1. A mutant β-glucosidase comprising an amino acid sequence having a sequence homology of at least 90% to the amino acid sequence of SEQ ID NO: 1, wherein the amino acid sequence having a sequence homology of at least 90% comprises mutations of amino acids corresponding to at least three amino acids selected from the group consisting of glutamic acid at position 39, aspartic acid at position 169, arginine at position 170, arginine at position 220, tyrosine at position 227, and glutamic acid at position 330 in the amino acid sequence of SEQ ID NO: 1.