Biomarkers in peripheral blood mononuclear cells for diagnosing or detecting lung cancers

摘要

Methods and compositions are provided for diagnosing or detecting a condition, e.g., lung disease in a mammalian subject by use of a micro-RNA expression level or an expression level profile of multiple miRNA in the peripheral blood mononuclear cells (PBMC) of the subject which is characteristic of COPD or NSCLC. Detection of changes in expression in specific miRNA biomarkers from that of a reference sample or miRNA expression profile are correlated with non-small cell lung cancer (NSCLC) and/or COPD and permit differentiation among healthy subjects, subjects with COPD and subjects with adenocarcinoma or squamous cell carcinoma.

主权项

1. A diagnostic kit comprising a set of PCR primer pairs and a labeled fluorogenic probe which generates a measurable signal, wherein each primer pair is capable of specifically complexing with, hybridizing to, or amplifying a single different miRNA in a set of miRNA, or a cDNA generated therefrom, the set consisting of hsa-miR-148a, hsa-miR-142-5p, hsa-miR-221, hsa-miR-let-7d, hsa-miR-let-7a, hsa-miR-328, hsa-miR-let-7c, hsa-miR-34a, hsa-miR-202, hsa-miR-769-5p, hsa-miR-642, wherein the set of PCR primer pairs consists of a PCR primer pair for each miRNA in the set of miRNA.