METHOD AND TEST KIT FOR RAPID DETECTION OF INSECTICIDE RESISTANCE IN AN INSECT DUE TO GLUTATHIONE-S-TRANSFERASE ENZYMES

摘要

THE PRESENT INVENTION RELATES TO A METHOD AND TEST KIT FOR RAPID DETECTING GLUTATHIONE-S-TRANSFERASE ENZYMES IN AN INSECT AND WHICH CAUSES INSECT RESISTANCE TO INSECTICIDES. IN PARTICULAR, THE PRESENT INVENTION RELATES TO A METHOD WHEREIN A SINGLE INSECT IS FIRST HOMOGENIZED TO RELEASE THE BODY FLUID CONTAINING THE GLUTATHIONE-S- TRANSFERASE ENZYMES AND THEN REACTING THE HOMOGENATE CONTAINING THE GLUTATHIONE-S- TRANSFERASE ENZYMES WITH A SUBSTRATE IN A WELL ON A MICRO-TITRE PLATE TO PRODUCE A VISUALLY DETECTABLE COLOUR. THE COLOUR IS AN INDICATION OF THE INSECT RESISTANCE OF THAT PARTICULAR INSECT. USING THIS METHOD, A LARGE NUMBER OF INSECTS CAN BE SCREENED IN A SHORT PERIOD OF TIME AND HENCE USEFUL FOR VECTOR CONTROL ACTIVITIES.