STRAIN OF FMD VIRUS Aphtae epizooticae OF TYPE A FOR CONTROLLING ANTIGENIC AND IMMUNOGENIC ACTIVITY AND FOR PRODUCTION OF BIOLOGICAL PREPARATIONS FOR DIAGNOSTICS AND SPECIFIC PROPHYLAXIS OF FMD OF TYPE A

摘要

FIELD: biotechnology.SUBSTANCE: characterised strain was isolated from diseased pigs and produced by serial passages on sensitive hetero- and homologous cell cultures and deposited in the collection of the FSBI "Federal Animal healthcare centre" under the registration number of FMD virus strain A No.2187/Kuti/2013 (production). The presented strain is reproduced in monolayer cell culture of porcine kidney (PK), passaged cell cultures of kidney of Siberian mountain ibex (SMIK-30), VPK-21 and IB-RS-2. During 18÷24 hours of incubation the virus yield in the said cell cultures reaches the values of 6.0÷7.0 lg TCD/cm. At high multiplicity of infection (1÷10 TCD/cell) causes TCIDafter 5 hours, while maintaining the original characteristics when passaging in cell cultures for 5 passages.EFFECT: invention can be used to monitor the antigenic and immunogenic activity and for producing biological products for diagnostics and specific prophylaxis of FMD of type A.6 tbl, 6 ex