Methods of treatment using glycopegylated G-CSF

摘要

The present invention provides a glycopegylated G-CSF that is therapeutically active and which has pharmacokinetic parameters and properties that are improved relative to an identical, or closely analogous, G-CSF peptide that is not glycopegylated. Furthermore, the invention provides methods for mobilizing hematopoiesis in a subject, particularly a subject who has received or will receive radiation or chemotherapy treatment. The methods and compositions of the invention can further be used to prevent, alleviate and treat the myelosuppressive effects such therapies.

主权项

1. A method for increasing stem cell production in a donor, said method comprising administering to said donor an amount of a covalent conjugate between a G-CSF peptide having the amino acid sequence according to SEQ ID NO: 1 and a poly(ethylene glycol) (PEG) group,
wherein said covalent conjugate comprises a structure according to the formulawherein
q is 0 or 1; and Sia-PEG has a structure according to the formula: in which n is an integer from 400 to 500, and wherein said amount is in a range from about 1 mg to about 20 mg or is a unit dosage form selected from 50 μg/kg, 100 μg/kg, and 200 μg/kg.