Insect cell line for production of recombinant glycoproteins with sulfated complex N-glycans

摘要

A transgenic insect cell line for production of recombinant glycoproteins possessing sulfated, complex N-glycans is provided.

主权项

1. A transgenic insect cell for the production of at least one protein of interest comprising sulfated complex N-glycans, said insect cell comprising at least one nucleic acid molecule encoding at least two modifying enzymes selected from the group consisting of:
i) —N-acetylglucosaminyltransferase II; ii) —N-acetylglucosaminyltransferase III; iii) —N-acetylglucosaminyltransferase IV; iv) -Beta 1,4-Galactosyltransferase; v) —N-acetylglucosamine-6-O-sulfotransferase; vi) -Galactose-3-O-sulfotransferase; and vii) —N-acetylglucosaminyltransferase V, said cell comprising at least nucleic acids encoding i) and v) or i) and vi) and optionally comprising at least one additional nucleic acid molecule encoding said at least one protein of interest, wherein said modifying enzymes catalyze the formation of sulfated complex N-glycans on said protein of interest.